DNA
Part:BBa_J70010:Design
Designed by: Julie Norville Group: Knight Lab (2008-05-09)
A BioScaffold Part (Uses PpiI), for offset see Part Design page
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 48
Design Notes
Can be used to cut at 0 offset from the BioBrick scar on either side, see BBF RFC15
TACTAGAG and TACAGAT represent sequences for BioBrick scars that are created when
two BioBrick parts are fused together.
^s represent restriction enzyme cut sites.
General Function
XXXXX XXXXX^TACTAGAG BioScaffold Part BBa_J70010 TACTAGAT XXXXX^XXXXX XXXXX^XXXXX ATGATCAC ATGATCTA^XXXXX XXXXX
Function when the right part is a protein part.
XXXXX XXXXX^TACTAGAG BioScaffold Part BBa_J70010 TACTAGAT GXXXX^XXXXX XXXXX^XXXXX ATGATCAC ATGATCTA^CXXXX XXXXX
What happens after cutting with MabI or PacI and purifying with either gel purification
or a Qiagen PCR cleanup spin column
General Case
XXXXX XXXXX^ ^XXXXX XXXXX^ ^XXXXX XXXXX
Case when right part is a protein part
XXXXX XXXXX^ ^XXXXX XXXXX^ ^CXXXX XXXXX
Source
designed