DNA

Part:BBa_J70010:Design

Designed by: Julie Norville   Group: Knight Lab   (2008-05-09)

A BioScaffold Part (Uses PpiI), for offset see Part Design page


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 48


Design Notes

Can be used to cut at 0 offset from the BioBrick scar on either side, see BBF RFC15

TACTAGAG and TACAGAT represent sequences for BioBrick scars that are created when

two BioBrick parts are fused together.

^s represent restriction enzyme cut sites.

General Function

XXXXX XXXXX^TACTAGAG  BioScaffold Part BBa_J70010 TACTAGAT XXXXX^XXXXX
XXXXX^XXXXX ATGATCAC                              ATGATCTA^XXXXX XXXXX

Function when the right part is a protein part.

XXXXX XXXXX^TACTAGAG  BioScaffold Part BBa_J70010 TACTAGAT GXXXX^XXXXX
XXXXX^XXXXX ATGATCAC                              ATGATCTA^CXXXX XXXXX

What happens after cutting with MabI or PacI and purifying with either gel purification

or a Qiagen PCR cleanup spin column

General Case

XXXXX XXXXX^                                                    ^XXXXX
XXXXX^                                                    ^XXXXX XXXXX

Case when right part is a protein part

XXXXX XXXXX^                                                    ^XXXXX
XXXXX^                                                    ^CXXXX XXXXX

Source

designed

References